In this study,mostexperimentsweretypicallyconductedatroomtemperature.theseon-packagelabelsareconventionallygeneratedfromHydrophilicflmpolymersorcoatingmaterialsincorporatingsingleormixedph-sensitivesyntheticdyes(Caoet al.,2019; Chen et al.,2019; Kuswandi & Nurfawaidi,2017),naturaldyes(maetal.,2017; Zhang et al.,2019),Uorescentdyes(Puligundla,Jung,& Ko,2012),ornanoparticles(mills&hawthorne,2017; Mills &Hazafy,2009(.theColorimetricAssay’ssensitivityistypicallycausedbythedissolutionofvolatilebasicNitrogencompoundsorco2inwatertrappedinflmortheenvironment(mills&skinner,2010).Nevertheless,thesensitivityoftheHydrophilicFLM-formingindicatormanageraffectedbychangesintemperatureorhumidity,specifcally when usedunderdifferentstorageconditions.anegligiblehumiditylevelinsidethepackagemaysuppresstheindicator’ssensitivityresultinginfalseindications(Kim,Lee,Lee,Baek,& Seo,2017),whichisundicationsintelligentpackaging.topreventfalseindication,there is a need toinvestigatealternativeHydrophilicmaterialsthatcanabsorbmoisturetoactasthesolidsupportmatrixforph-sensitivedyeandFacilitatetheacid-basereactionresultingfromthedissolutionofvolatilegas。HydrogelsarehighlyHydrophilicmaterialsthathaveamajorroleinimmobilizingandsupportingtheindicator.theyalsohaveanExcellentmoisture-holdingabilitytoenhancethephindicator’ssensitivitytochemicalsdevelopedduringfoodspoilage.tothebestofourknowledge,limitedresearchisavailableregardingtheHydrogelindicator’ssensitivitybasedonTVB-ncompoundgenerationfrommeatspoilage.hencethesimplefabricationofHydrogelindicatorsthatexhibitacolorresponsetoTVB-ncompoundsproducedbythedecompositionofproteininmeatunderlowstoragetemperatureisanexcellentandurgentlyrequired approach。alginateisanon-toxicandlow-costpolysaccharidethatpossessesgellingabilityVIAcrosslinkingusingionicinteractionswithdivalentcationssuchasca2+(Kaklamani,Cheneler,Grover,Adams,& Bowen,2014).themechanicalStrengthcanbeenhancedbyincreasingthealginateconcentrationorthecrosslinkingdensity(auGST,Kong,&Mooney,2006).methylcelluloseiscommonlyusedasaviscosityenhancingpolymerinfoodandPharmaceuticals(liangetal.,2004)。alginateandmethylCellulosewerefoundtoblendcompatiblywitheachOther(BanerJee,Singh,Bhattacharya,& Chattopadhyay,2013; ライアンet al.,2004).blendingmethylcellulosewithalginateHydrogelcouldimprovethegelviscosityandstackabilityofthe3dbioprintingoutput(Li,Tan,Leong,& Li,2017).Therefore,blendingalginatewithmethylcellulosecouldenhancegelstrength,whichisanimportaaathrequired for further application。In this research,HydrogelsweredevelopedfromSodiumalginateblendedwithmethylcelluloseviatheexternalgelationmethod。Hydrogelindicatorsweresubsequentlypreparedbyincorporatingasingle pH-sensitive dye,bromothymol blue. Herein,weaimedtoevaluatethephysicalproperties,mechanical strength,morphologyoogycomposite hydrogel,andthepreparedhydrogelindicator.toinvestigatetheeffectofHumidityontheColorimetricsensitivity,the experimentwascarriedoutinanAlkaline(NH3)environment at different RH. Weperformedapreliminaryinvestigationonthehydrogelindicator’sColorimetricsensingabilitytomonitormincedporkspoilageresultingfrom TVB-N in packaging,to test its practical application。
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