Serum separation gel product manual
[product name]
generic name: serum separation gel (radiation)
[Specification] 25KG/ a barrel []
product standards, product performance] [this product is a colorless transparent colloid, a faint smell, no impurities; a physiologically inert, insoluble in water, relative the proportion of 1.045-1.065g/cm3, the viscosity of the 10-30 Wan Li PA, volatile ≤ 1.5%, excellent thixotropy and isolation performance, other performance see product testing report.
[] this product is main component containing a hydrophobic organic polymers, silica powder, thixotropic agent, stabilizer and so on;
[] this product suitable scope for the manufacture of disposable vacuum blood collection tube and injection type vessel, as clinical test for preparing high quality serum, blood sample preservation, transfer, transfer of the necessary materials.
[] serum separation principle generally accounted for about 1.030, clot accounted for about 1.092, the separation gel proportion is 1.045 ~ 1.065. Separation gel has good thixotropic properties and isolation performance. When the separation gel and solidification of blood in the same tube centrifugation, under the action of centrifugal force, the separation gel intermolecular hydrogen bond breaking into low viscosity fluid, because of its specific gravity is between blood cells and serum,Separation gel during centrifugation will gradually accumulate in the serum and blood clots. When the disappearance of external centrifugal force, intermolecular hydrogen bonding to associating the formation of network structure, the separation gel to become colloid of high viscosity, forming an isolation layer will be separated from the serum and blood clots. [Methods]
1 glue added: the machine or manual feeding;
(1) with automatic, semi-automatic and glue machine glue, first separated the glue machine hopper, the colloid leveling, covered hopper, you can start charging. Low temperature in winter, conditions may be appropriate to heating, oil bath, water bath or by both electric heating,Control the temperature of 30-60 ℃. (2) the manual feeding, the colloidal injection feeder, feeding head is arranged at the bottom of the tube, slowly feeding rod, with colloidal slowly flows into the test tube, lifting and material, and add enough colloid, carefully took the feeder.
2 air bubble: the automatic gluing machine, tube upright glue will join the colloid placed 8-12 hours, and then sealed vacuum tube, about 70% or so no bubbles. Manual gluing may cause large amounts of gas into a vacuum, there will be a lot of bubbles. Bubbles appear, suggest using a centrifuge in 800-1200g centrifugal force, the centrifugal exhaust gas in 2-5min.The centrifugal force and the centrifugal time air bubble needs and gum, colloidal transfer process into the air much about, recommend the use of process based on the actual situation of adjustment.
centrifugal air bubble can be 2H with glue, can also be carried out in vacuum 1h. Separation gel and coagulant, anticoagulant used at the same time, suggest to join the separation gel, and then air bubble, adding coagulant and anticoagulant, then vacuum. This can make the separation gel effect of coagulant, anticoagulant minimum.
static: both the gluing process, or vacuum, centrifugal process, will lead to temporarily reduce the glue thixotropy separation, suggestions in with glue,From the heart, will complete the
tube upright standing 1H above, to restore the easy separation gel thixotropy. Vacuum after the placement of 1H, is conducive to the colloid air fully released.
4 this product can and coagulant, anticoagulant (EDTA salt, salt used in conjunction with heparin). Centrifugal conditions:
5 (1): in the blood serum preparation fully solidified, in a centrifuge with 1800-2200g centrifugal force of serum 8-10min can be prepared with high quality.
(2) preparation in blood plasma: after mixing, reverse 5-8, anticoagulation can be fully after centrifugation. Centrifugal conditions.
6 sterilization: the company is divided into two separation gel,A common separation gel, sterilization can not tolerate cobalt 60 irradiation; a radiation separation gel, can use Cobalt 60 irradiation sterilization. Recommendations in 8-40KGY dose range sterilization. The irradiation dose to the initial colony number determination.
[] dosage of each tube suggested adding separation gel for the amount of 0.8-1.2g (0.75-1.1ml). For 16mm diameter plastic pipe, plus amount of glue should be more than 1.0-1.2g. To determine whether the addition amount of glue enough standard is normal after centrifugation, gel (isolation layer) thickness of 6mm.
[] without contraindications. [note]
the separation gel for the patented product, unique composition,Please do not mix and other suppliers of products, so as to avoid the products of incomplete, causing unnecessary losses to you;
recommended temperature separation gel using range is -20 ~ 90 ℃. This product with the change of environmental temperature, the viscosity will change significantly. The ambient temperature is too low, can lead to difficulties with glue. Recommendations in the environment temperature is below 15 ℃ water bath, can be heated to 60 ~ 70 ℃, lower viscosity, improve efficiency and adhesive; room temperature with glue when
"drawing" phenomenon, can increase with temperature, or in vibration mode will wire broken;
This product after Taka Shinzora treatment, basic no colloid air entrainment, is generally not recommended in the transfer,Gluing process for mixing, because stirring easily lead to colloid with a lot of air;
This product into the test tube, under a vacuum condition through the inverted test more than 20 consecutive days at 60 ℃, colloidal plane flow distance is smaller than 5mm. Can guarantee the container in 60 ℃ continuous transport 20 days not more than 5mm flow, normal use will not affect the blood collection. Even so, we still recommend that, in a long time in the process of transportation, including separating gel tubes not inverted;
in recommended not to directly touch the separation gel, gel stick to the skin can use first clean sheet away with waste newspaper, scrub out most, then DMC,D4 organic solvent cleaning, or mixed with sawdust detergent powder, detergent, then rinse with water.
cleaning separation gel hopper: the separation gel replacement of different manufacturers, or placing a long time not exposed in the air and feeding back into the bucket, the use of the former should clean up. Cleaning method: first use stainless steel shovel colloidal hopper around surface out clean, then brush dipped in DMC, D4 and other solvent cleaning, and then use the cloth dipped in a solvent wipe clean. The feeding tube should be cleaned to prevent pollution, colloid.
[warning and prevention]
1 when serum separation gel with impurities,, abnormal smell,Abnormal color and exceed the term of validity, the prohibition of the use of.
in 2 as the inert material, safety, non-toxic.
3. this product should not be mixed with other manufacturers and product use, may lead to the emergence of the phenomenon of mixed colloid layer, opacity etc..
4. beyond the recommended storage temperature limits may damage the separation gel quality.
[graphic] installation instructions or not applicable.
[instructions or graphic] please read the instructions carefully before using and according to the instructions.
RCF and centrifugal velocity (RPM) of the conversion relationship:
type: the R in - in centrifugal radius, representatives from the centrifuge axis to the bottom of the tube pitch
, expressed in cm.Relative centrifugal force (RCF) proposal and centrifugal time see table:
rpm=
in in in in in in in in in in RCF * 105
1.12 * r
[products] is not suitable for the maintenance and repair methods.
[special storage conditions and methods] unless otherwise indicated in packaging and labeling, general recommendations sealed preservation, storage temperature is 0-40 ℃ (32-104 ° F), avoid direct exposure to air and sunlight.
[duration] 24 months
[product standards should be in the instructions indicated no other content]
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