2.3. Disruption of MdTIC reduces apple freezing tolerance。<br>Because MdTIC acts as a downstream target of MdMYB88 and MdMYB124, we determined if MdTIC plays a role in apple freezing stress tolerance. We first examined MdTIC expression in MdMYB88 transgenic plants under cold treatment. As shown in Fig. 3A, MdTIC expression was downregulated in MdMYB88/124 RNAi plants, but upregulated in MdMYB88 overexpressing plants, suggesting positive regulation of MdTIC by MdMYB88 and MdMYB124 in response to cold.
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