肝素锂产品手册【产品名称】肝素锂【包装规格】塑料瓶，10g/每瓶，20

肝素锂产品手册
【产品名称】肝素锂

【包装规格】塑料瓶，10g/每瓶，20g/瓶，50g/瓶

【产品标准】Q/WHDS 0202-2012

【产品性能】 白色无定型粉末，无嗅，易溶于水，易吸潮，平均分子量为15000，常温下稳定。本公司肝素锂效价≥150IU/mg，无水效价≥160IU/mg。其它指标参照肝素钠原料药标准予以控制。

【主要用途】本品专用于血液体外抗凝，为临床血液检验的抗凝添加剂，在临床血液采集和检验过程中进行血液标本的前处理（抗凝处理）。

【适用范围】本品适用于临床生化学检查和急诊生化检查的血液标本的采集与抗凝，也适用于部分血液流变学项目的血液标本采集与抗凝。
在临床检验测定血液中离子含量时，推荐使用肝素锂作为抗凝剂，因为它最不容易对其它离子的测定产生干扰。
本品不是药品，不能作为注射剂使用，禁止直接注入人体和动物体内。

【使用方法】本品用去离子水（蒸馏水）溶解，配制成一定浓度和含量的溶液，然后加入采血管中。
一．溶液配制方法及程序：
1.准备事项：电子天平（万分之一）、容量瓶（100ml、200ml，500ml，1000ml，依据需要选用）、烧杯、去离子水（蒸馏水）、玻璃棒、洗瓶等。
2.称量：用电子天平准确称量事先确定的肝素锂粉末若干，精确到万分之一，然后仔细将肝素锂粉末倒入容量瓶或者烧杯中，用洗瓶小心将附着在容量瓶或者烧杯口及壁上的肝素锂粉末冲入瓶或杯中；特别注意容量瓶和烧杯要清洗干净，不能有杂质、油污、细菌及氧化物等存在。容量瓶和烧杯的清洗请参照《化验员操作手册》中相关内容。
3.溶解：本品可用蒸馏水、去离子水进行溶解。可以先在烧杯中加入一定量的去离子水溶解，边加水边用玻璃棒缓慢搅拌，待全部溶解后转入容量瓶中。也可直接用容量瓶溶解。用烧杯溶解时要特别注意溶解过程中用水量的控制，要给后面清洗玻璃棒、烧杯等用具上沾附的肝素留有足够的容积，否则会造成超容导致肝素浓度降低。
4.定容：待肝素锂全部溶解后，眼睛与容量瓶刻度线保持水平状态，用洗瓶缓慢沿容量瓶口壁加入去离子水，待凹形液面底与刻度线在一条线上即可。
5.混匀：用容量瓶塞密封容量瓶口，一手拿住容量瓶上部并用手指压住塞子，一手拿住容量瓶底部，小心颠倒混匀5-8次。
6.检查和确认：仔细检查肝素锂重量称量及容量瓶容积等关键因素，重新计算肝素锂有效含量，由主管确认无误即可。
二．添加方法：
1.机器加入：
（1）清洗：机器使用前要仔细将机器上的容器和输送管道清洗干净，可先用去离子水冲洗，然后用80%浓度酒精清洗容器和管道3次，然后再用99%以上乙醇清洗容器和管道，用热风吹干酒精即可。保证没有杂质、细菌和水附着在容器和管壁，避免对肝素锂造成污染。
（2）机器调整：依据将要生产的采血管容积确定需要加入肝素锂数量或者体积数，调整机器流量调节器到合适的数值，加入10-100支试管，并抽检加入剂量是否符合要求。溶液加入的容许误差为±10%。

2.人工加入：
（1）清洗容器和工具。清洗准备用于分装肝素锂溶液的容器和移液器，方法同机器的清洗方法。
（2）分发肝素溶液和工具：将肝素锂溶液分装到清洗好的容器中，和经过清洗、调整的的移液器一起分发给作业人员。注意移液器的刻度要调整好，保证加入的溶液体积达到规定要求。
（3）加液：采用移液器将肝素锂溶液加入采血管中。对每一个作业人员的加入量进行确认。过程中随时对加入量进行抽查。加入量容许误差范围为±10%。
三．烘干：可以采取热风鼓风烘干，也可以采用干燥箱、烘房等烘干。热风烘干温度在50-80度，烘干时间在10-300秒。鼓风干燥箱、烘房烘干在40-60度，烘干时间在2-6小时。烘干时间与肝素锂溶液的容积、烘干温度以及鼓风速度（干燥箱排风能力）等因素有关。特别注意鼓风速度不能太大，以免造成肝素锂溶液被吹到采血管外面。
肝素抗凝通常以溶液状态为最佳，但有些用户需要烘干的肝素锂试管。

【添加剂量】真空采血管行业标准中推荐的剂量范围为每毫升血液10USP（9.4IU）-30USP（28IU）单位肝素锂。微量采血器（末稍采血）抗凝可以小于15USP（14IU）每毫升血。本公司推荐一次性静脉真空采血管采用15-20IU肝素锂抗凝一毫升血液，末梢血抗凝采用8-10IU抗凝一毫升血液。
肝素效价单位：IU/mg。即每毫克含有肝素的有效数量。
示例表：常用肝素锂溶液配制及添加剂量表
采血管规格（ml） 2 3 4 5 6 7 8
肝素添加剂量（IU） 30 45 60 75 90 105 120
对应的肝素锂重量（mg） 0.2 0.3 0.4 0.5 0.6 0.7 0.8
添加剂体积（ul） 20 30 40 50 60 70 80
肝素溶液浓度（g/l） 10 10 10 10 10 10 10
说明：1.肝素锂添加剂量按照每毫升血液添加15IU肝素计算；
2.肝素锂效价以150IU/mg计；
3.实际生产过程中肝素添加剂量可以15-25IU灵活掌握，肝素效价以检验报告上的实际数据为准。计算方法相同。
4.计算方法为：
（1）对应的肝素锂重量（mg） = 肝素锂添加剂量（IU）/肝素锂实际效价（IU/mg)。
(2)浓度（g/l）= 对应的肝素锂重量（mg）/添加剂体积（ul）。特别注意单位的变换即可，mg和ul同时换成g和ml，得出的百分比为100ml水中加入的肝素锂的克数，最后ml要换成l，需要分子分母同时乘以10，得出的才是每升水中需要加入的肝素锂的重量。

【注意事项】
 为保证血液抗凝充分，肝素盐试管采血完成后必须尽快颠倒混匀5-8次，特别在采血环境温度高于25℃时，血液与肝素锂的混匀必须及时充分，否则易导致血液凝固或者局部凝血。
 肝素抗凝非不可逆抗凝，故肝素锂抗凝试管采血后应在6h内完成检验，否则可能导致检验结果出现误差。
肝素可能参与细胞酶和离子的代谢，因而肝素的使用量可能影响检测结果。肝素的添加剂量应确保标本全部和局部的充分抗凝，从而保证血浆的大部分指标6h内可重复，尤其是AST、ALT、TBIL、DBIL、GGT等敏感指标。
肝素锂可以与分离胶同时使用，抗凝效果、管壁硅化、离心条件、分离胶质量等都会影响血液分离效果。建议与本公司生产的血液分离胶配合使用，可以得到高质量的血浆标本。
加入到采血管后辐照灭菌建议：使用γ射线照射，剂量8-25kGy。辐照剂量可以初始菌落数确定。

【警告和预防】
 当肝素锂出现杂质异物、异常气味、颜色以及超过有效期限时，禁止使用。
当肝素锂溶液出现混浊时，可能是肝素锂溶液已染菌或变质，请勿继续使用。
本品为生物制剂，安全、无毒、无害。

【肝素抗凝原理】肝素系自动物肠黏膜中提取的含有硫酸基团的粘多糖，是一种带有强大的负电荷的生物活性物质，肝素在血液中具有激活抗凝血酶Ⅲ灭活丝氨酸蛋白酶的作用，从而阻止凝血酶的形成，并有阻止血小板聚集等多种抗凝作用。它对凝血过程的三个阶段均有影响，在体内外均有抗凝作用，可延长凝血时间、凝血酶原时间和凝血酶时间。

【使用说明或图示】使用前请仔细阅读本产品手册。不清楚事项请与本公司联系。

【产品维护和保养方法】不适用。

【储存条件】本品可在阴凉干燥处密封保存，避光，不能受潮。本品的水溶液可在无菌状态下0-4℃密封保存，最长保存期限不要超过7天。建议本品水溶液即配即用，不要以溶液状态长时间存储。

【有效期限】三年

【其它事项】常见单位换算
1.重量单位：1g = 1000 mg；1kg = 1000 g；
2.体积单位：1ml = 1000 ul，1l = 1000 ml；
3.效价单位：1IU = 1.064 USP 。

【生产企业名称】武汉德晟化工科技有限公司

【注册地址】武汉市东湖风景区龚家岭鲍家湾88号

【生产地址】武汉市东湖风景区龚家岭鲍家湾88号

【联系方式】
电话：（+)86 027-86576982 传真：（+）86 027-86565322

【售后服务单位】
武汉德晟化工科技有限公司
服务专线：（+）86 02786565322 （+）86 13397176588

結果 (英文) 1:

Lithium heparin product manuals
[product name] lithium heparin

【Package】 plastic bottle, 10g / bottle, 20g / bottle, 50g / bottle

[Product standard] q / whds 0202-2012

[Performance] white amorphous powder, odorless, soluble in water, easy to absorb moisture, the average molecular weight of 15,000, room temperature stability. The Company lithium heparin titer ≥ 150iu/mg, anhydrous titer ≥ 160iu/mg. Other indicators heparin API reference standard be controlled.

The main purpose of this product is designed for in vitro blood anticoagulated blood tests for clinical anticoagulant additives,Blood collection and testing in the clinical course of the pre-treatment blood samples (anticoagulated).

The scope of application of the product is suitable for clinical biochemical tests and emergency biochemistry blood specimen collection and anticoagulation, also applies to the part of the rheology of blood specimen collection and project anticoagulation.
In clinical analysis laboratory ion content in the blood, it is recommended to use lithium heparin as an anticoagulant, because it is not easy for most other ions interfere with the determination.
This product is not a drug and can not be used as an injection, prohibits direct injection humans and animals.

【Use of this product with deionized water (distilled water) dissolvedPreparation of a certain concentration and amount of the solution, and then added to blood collection.
A. Solution preparation methods and procedures:
1. Preparation: electronic balance (parts per million), flask (100ml, 200ml, 500ml, 1000ml, based on the need to use), beakers, deionized water (distilled water), glass, wash bottles.
2. Weighing: accurately weighed using an electronic balance lithium heparin powder pre-determined number, accurate to millionth lithium heparin powder was then carefully poured into the flask or beaker, bottle carefully adhered to the flask Or the wall of the beaker mouth and lithium heparin powder into a bottle or cup;Special attention volumetric flasks and beakers to be cleaned, can not have impurities, oil, bacteria and oxides exist. Volumetric flasks and beakers cleaning refer to "Laboratory Technician Operating Manual" relevant content.
3 solution: It can be distilled, deionized water to dissolve. Can be added to a certain amount in a beaker of deionized water, while stirring slowly with a glass rod waterside added until all dissolved into the flask. Can also be directly dissolved in a volumetric flask. Beaker should pay special attention when dissolved in water dissolution process control, to give back clean glass rod, beakers and other utensils contaminated with heparin sufficient volume,Otherwise it will create super-capacity led to heparin concentration decreased.
4. Constant volume: to be dissolved lithium heparin whole, the eyes and the flask scale line remains horizontal, along with a bottle capacity of the bottle wall slowly adding deionized water until the bottom of the concave surface in a line with tick marks can be.
5. Mixing: with capacity of cork sealed bottle capacity, hand hold the upper flask stopper with your fingers in one hand and hold the bottom of the flask, carefully mix by inversion 5-8 times.
6. Checked and confirmed: Carefully check weighing and weight heparin lithium volumetric flask and other key factors, lithium heparin recalculated effective content, can be determined by the competent confirmation.
II. Add Method:
1 Machine added:
(1) Cleaning: machine carefully before using the machine clean containers and pipelines can be rinsed with deionized water, and then cleaned with 80% alcohol containers and pipes three times, and then washing with 99% ethanol, containers and pipes, hot air drying alcohol can be. To ensure that no impurities, bacteria and water attached to the container and the wall, to avoid polluting lithium heparin.
(2) to adjust the machine: According to blood collection to be produced lithium heparin need to add volume to determine the quantity or volume number, flow regulator to adjust the machine to the appropriate value by adding 10-100 test tubes,And sampling added dose compliance. Solution was added to the tolerance of ± 10%.

2. Artificial added:
(1) clean containers and tools. Ready for dispensing a cleaning solution of lithium heparin containers and pipettes, the same way the machine cleaning methods.
(2) distribution of heparin solution and tools: The lithium heparin solution was dispensed into a cleaned container, and after washing, the adjustment of the pipette with the distributed operating personnel. Note that the scale necessary to adjust the pipette to ensure that the volume of solution added to meet the requirements.
(3) Dosing: The pipette solution was added lithium heparin blood collection tubes.For each worker the amount of added confirmation. Process at any time to conduct random checks on the added amount. Added amount allowable error range of ± 10%.
Three. Drying: You can take the hot air blast drying oven can also be used, such as drying drying room. Hot air drying temperature is 50-80 degrees, drying time is 10-300 seconds. Drying oven, drying room drying at 40-60 degrees, drying time is 2-6 hours. Drying time and the volume of heparin lithium solution, drying temperature and the blast air velocity (oven exhaust capability) and other factors. Special attention blast speed can not be too large, so as to avoid a solution of lithium heparin blood collection tube was blown out.
Heparin solution state is usually the best, but some users need to be dried lithium heparin tubes.

[Amount] additive Vacutainer industry standard recommended dose range per milliliter of blood 10usp (9.4iu)-30usp (28iu) units of heparin lithium. Trace lancing devices (peripheral blood) anticoagulation can be less than 15usp (14iu) per milliliter of blood. The Company recommends using disposable Vacutainer vein 15-20iu lithium heparin anticoagulant one milliliter of blood, peripheral blood anticoagulant anticoagulant using 8-10iu one milliliter of blood.
Heparin potency unit: iu / mg. Per mg of heparin-containing effective amount.
Sample table:Common lithium heparin solution preparation and additives Scale
cartridge specifications (ml) 2 3 4 5 6 7 8
additive amount of heparin (iu) 30 45 60 75 90 105 120
corresponding lithium heparin weight (mg) 0.2 0.3 0.4 0.5 0.6 0.7 0.8
additive volume (ul) 20 30 40 50 60 70 80
heparin concentration (g / l) 10 10 10 10 10 10 10
Instructions: 1. lithium heparin per milliliter of blood in accordance with the amount of additives added 15iu heparin calculation;
2 lithium heparin titers 150iu/mg meter;
3. actual production process, the amount of additive can heparin 15-25iu flexibility,Heparin potency to the actual data on the inspection report shall prevail. Calculated in the same way.
4 is calculated as:
(1) corresponds to the weight of lithium heparin (mg) = amount of the additive lithium heparin (iu) / Actual lithium heparin potency (iu / mg).
(2) concentration (g / l) = weight of the corresponding lithium heparin (mg) / additive volume (ul). Special attention to the unit conversion, mg, and ul replaced both g and ml, the percentage of derived 100ml water added to the number of grams of lithium heparin, and finally be replaced ml l, 10 to the numerator and denominator are multiplied, derived The water is to be added per liter of lithium heparin weight.


[Note]In order to ensure adequate blood anticoagulant, heparin blood collection tubes after completion salt mix by inversion 5-8 times as soon as possible, particularly in the blood when the ambient temperature is above 25 ℃, blood mixed with lithium heparin must timely and adequate, otherwise easily lead to blood clotting or partial clotting.
Irreversible non-heparin anticoagulant, so lithium heparin anticoagulant blood collection tubes should be within 6h after the completion of the inspection, the test results may cause errors.
Heparin may be involved in cellular metabolic enzymes and ions, and therefore the use of heparin may affect test results. The additive amount of heparin should ensure full and partial specimens anticoagulation,Thus ensuring most of the indicators of plasma can be repeated within 6h, especially ast, alt, tbil, dbil, ggt and other sensitive targets.
Lithium heparin gel can be used simultaneously, anticoagulant effects, wall silicification, centrifugation conditions will affect the quality of separating gel blood separation. Proposal with the company's blood separation gel with the use of high quality can be obtained plasma samples.
Added to the blood collection after irradiation sterilization Recommendation: Use the γ-ray irradiation, the dose 8-25kgy. The initial number of colonies radiation dose can be determined.


WARNING and prevention] appear when lithium heparin impurity foreign body, unusual odor, color, and over the validity periodProhibited.
When lithium heparin solution appears cloudy, it may be a solution of lithium heparin contamination or deterioration have been, do not continue to use.
This product is a biological preparation, safe, non-toxic, harmless.

【Principle】 heparin heparin Department extracted from animal intestinal mucosa group containing sulfuric acid mucopolysaccharide, is a strong negative charge with bioactive substances in the blood with heparin activates antithrombin Ⅲ inactivation of serine proteases, thereby preventing the formation of thrombin, and prevent platelet aggregation and other anticoagulant effect. The three stages of the coagulation process it affects both have anticoagulant effects in vivo prolong clotting time,Prothrombin time, and thrombin time.

[Instructions or icon] Please read this before using the product manual. Unclear issues please contact the company.

[Product care and maintenance methods] does not apply.

【Storage product can be sealed and stored in a cool, dry place, away from light, not damp. This product is available in a sterile aqueous solution of 0-4 ℃ sealed state, the longest retention period should not exceed 7 days. Recommended that the product solution that is equipped to use, do not store in a solution state for a long time. [Effective date]


[three] other matters common unit conversion
1 unit of weight: 1g = 1000 mg; 1kg = 1000 g;
2.Volume units: 1ml = 1000 ul, 1l = 1000 ml;
3. Potency units: 1iu = 1.064 usp.

[Manufacturer name] Wuhan De Sheng Chemical Co., Ltd. Registered Address]

[Bao Jia Wan Gong Jialing East Lake Scenic Area No. 88

【Address】 Jia Wan Bao Gong Jialing East Lake Scenic Area No. 88

[Contact]
Phone: () 86027-86576982 Fax: () 86027-86565322


[Service Unit]
Wuhan De Sheng Chemical Co., Ltd. Hotline: () 8602786565322 () 8613397176588

結果 (英文) 2:

Lithium heparin product manual
[product name] heparin lithium

[packing] plastic bottle, 10g/ bottle, 20g/ bottle, 50g/ bottle

[] Q/WHDS 0202-2012

[product standards, product performance] white amorphous powder, odorless, soluble in water, easy absorption of moisture, the average molecular weight is 15000, and stability at room temperature. The company lithium heparin potency ≥ 150IU/mg, ≥ 160IU/mg anhydrous titer. Other indicators of heparin sodium API reference standard to control.

[] this product is mainly used for extracorporeal blood anticoagulant, additive for anticoagulation clinic blood test,Pretreatment of blood specimens in clinical blood collection and testing process (anticoagulant treatment).

[application] this product is suitable for collection and anticoagulant in clinical biochemistry and biochemistry examination of blood samples, blood samples were collected and anticoagulation is also applicable to the blood rheology project. In the determination of ion content in the blood of clinical laboratory, recommend the use of lithium heparin as an anticoagulant, because it's not easy for other ions generated by interference.
This product is not a drug, not as injection, to prohibit the direct injection in human and animal. [Methods]

using this product with deionized water (distilled water) dissolved,To prepare a solution of certain concentration and content, and then joined the mining vessel.
a solution method and program:
1 Preparation: electronic balance (1/10000), volumetric flask (100ml, 200ml, 500ml, 1000ml, on the basis of the need to use), beaker, deionized water (distilled water), glass rod, the washing bottle etc..
2 weighing: determine beforehand with the electronic balance weighing heparin lithium powder several, accurate to 1/10000, and then carefully heparin lithium powder into the volumetric flask or beaker, wash bottle carefully attached heparin lithium powder in a volumetric flask or beaker and wall into the bottle or cup;Pay special attention to the volumetric flask and the beaker to clean, can not have impurities, dirt, bacteria and oxide. The cleaning bottle and jar please refer to "relevant content." in the operation manual.
3: This product can be dissolved were dissolved in distilled water, deionized water. Deionized water solution can add a certain amount of first in the beaker, adding water and stirring with a glass rod slowly, after it is dissolved completely into the volumetric flask. Can also be directly used in volumetric flask dissolved. Special attention should be paid to the beaker dissolve in water control during dissolution, to give back, cleaning glass beaker and other appliances attached on the heparin with enough volume,Otherwise it will cause the super capacity leads to decrease in the concentration of heparin.
4 volume: the lithium heparin completely dissolving, eyes and volumetric flask scale line to maintain the level of state, with the bottle slowly along the bottle wall volume deionized water is added, the concave surface and the bottom of the scale lines in a line.
5 mixing: sealing capacity bottleneck capacity bottle stopper, one hand holds the bottle upper and fingers suppress plug, one hand holds the bottle bottom upside down, careful blending 5-8.
6 check and confirmation: carefully check the key factors of heparin lithium weighing and volumetric flask volume, recalculate the lithium heparin effective content, by the competent confirmation can be.
two. Adding method:
The 1 machines to join:
(1) cleaning: the machine carefully before use the cleaning container on the machine and pipeline clean, can be rinsed with deionized water, then with 80% concentration alcohol to clean the container and pipe 3 times, and then use the above 99% ethanol cleaning vessels and piping with hot air dryer, alcohol can be. Ensure the adhesion of the container and pipe wall has no impurities, bacteria and water, avoid pollution to the heparin lithium.
(2): on the basis of machine adjustment will be the production of blood volume determination requires the addition of heparin lithium quantity or volume number, adjust the machine flow regulator to proper value, add 10-100 tubes,Check whether it meets the requirements of accession and dose. Allowable error solution added is ± 10%.

2
(1) Manual: clean containers and tools. Cleaning preparation container for packing lithium heparin solution and the pipettes, cleaning method with the machine method.
(2) distribution of heparin solution and tools: lithium heparin solution subpackage to container cleaning and good, and after cleaning, adjusting pipette with the distributed to workers. Note the Graduated pipettes to adjust the volume of solution, which added to the required.
(3) plus liquid: using pipette will lithium heparin solution into the vessel.To confirm the amount of each one of the operations staff. At any time in the process of adding amount of checks. Amount allowable error range is ± 10%.
three. Drying: can take hot blast drying, can also be used for drying oven, drying room drying. Hot air drying temperature of 50-80 degrees, the drying time in 10-300 seconds. Drying oven, drying room drying in 40-60 degree, the drying time: 2-6 hours. The drying time and lithium heparin solution volume, drying temperature and blast velocity (drying oven exhaust ability) and other factors. Pay special attention to blast the speed can not be too large, so as not to cause lithium heparin solution was blown into the vessel.
Heparin is usually in solution state is the best, but some users need lithium heparin tube drying.

[] vacuum dosage dose range recommended industry standards for vessels in per milliliter of blood 10USP (9.4IU) -30USP (28IU) units of heparin lithium. Trace blood (peripheral blood) anticoagulation can be less than 15USP (14IU) per milliliter of blood. Our company recommend intravenous vacuum vessel using 15-20IU lithium heparin anticoagulation one ml of blood, peripheral blood anticoagulated with 8-10IU anticoagulation one milliliter of blood.
heparin potency unit: IU/mg. Effective number per mg containing heparin.
sample table:Commonly used lithium heparin solution and additive scale
vessel specifications (ML) 234567 8
heparin dosage (IU) heparin lithium weight of 3045607590105 120
corresponding to the (mg) 0.2 0.3 0.4 0.5 0.6 0.7 0.8
additive volume (UL) of 203040506070 80
heparin solution concentration (g/l) of 101010101010 10
Description: 1 lithium heparin dosage according to the calculation of added heparin per milliliter of blood 15IU;
2 lithium heparin potency as 150IU/mg;
3 heparin in actual production process can be flexibly added dose of 15-25IU,The actual data of heparin potency to inspection report on the subject. The same calculation method.
4 Calculation Methods:
(1) lithium heparin weight corresponding to (mg) = lithium heparin dosage (IU) / lithium heparin actual titer (IU/mg).
(2) concentration (g/l) of heparin lithium weight = corresponding (mg) / additive volume (UL). Pay special attention to transform unit, Mg and UL into G and ml, the percentage of heparin lithium 100ml by adding the number of grams, finally ml to replace the L, also need the numerator and denominator multiplied by 10, that is the lithium heparin per liter of water needs to be added to the weight of the. [note]


In order to ensure sufficient blood in anticoagulation, heparin salt test-tube blood must be completed as soon as possible to reverse the blending 5-8 times, especially in the blood temperature higher than 25 ℃, blood and lithium heparin mixing must be full and timely, otherwise easily lead to blood coagulation or local coagulation.
in heparin anticoagulation is not reversible, so lithium heparin anticoagulation tube blood collection should complete the inspection within 6h, otherwise it may lead to errors in test results.
heparin may be involved in cell enzyme and ion metabolism, so the use of heparin may influence the detection results. Add a dose of heparin should ensure that all samples and local full anticoagulation,To ensure that 6h most of the indexes of plasma can be repeated within, especially AST, ALT, TBIL, DBIL, GGT and other sensitive index.
lithium heparin with separation gel can be used at the same time, anticoagulant effect, tube wall silicification, centrifugal conditions, the separation gel quality will influence the separation of blood. With the use of blood separating gel and produced by our company, can get the plasma samples of high quality.
added to the vessel after irradiation sterilization recommendations: the use of gamma ray irradiation, dose 8-25kGy. Radiation dose can be the initial colony number determination. []


warning and prevention in lithium heparin appeared when impurities, abnormal odor, color and exceed the valid period,The prohibition of the use of. When lithium heparin solution turbidity, may be lithium heparin solution has contamination or deterioration, please do not continue to use.
This product is a biological agent, safe, non-toxic, harmless. Containing sulfate groups extracted intestinal mucosal automatic

[principle] heparin anticoagulant heparin in the mucopolysaccharide, with a strong negative charge of bioactive substances, activated antithrombin inactivation effect of heparin with serine protease in the blood, to prevent clotting enzyme formation, and prevent platelet aggregation. A variety of anticoagulant effect. Its impact on the three stages of coagulation process, has anticoagulant effect in vivo, can prolong the clotting time,Prothrombin time and thrombin time.

[instructions or graphic] before use, please read this manual carefully. Unclear matters please contact the company.

[products] is not suitable for the maintenance and repair methods.

[] this product storage conditions can be sealed and stored in a cool dry place, avoid light, not damp. The product of the aqueous solution in aseptic conditions 0-4 ℃ sealed, the longest duration of not more than 7 days. Recommend this product solution prepared in solution state, not long time storage.

[valid] three years

[] other matters of common unit conversion
1 unit of weight: 1g mg = 1000; 1kg = 1000 g;
2A unit of volume: 1ml = 1000 UL, 1L = 1000 ml;
3 titer unit: 1IU = 1.064 USP.

[manufacturer name] Wuhan De Sheng Chemical Technology Co., Ltd

[registered address] Wuhan East Lake scenic area Gong Ling Bao Chichiawan 88

[address] production of Wuhan East Lake scenic area Gong Ling Bao Jia Wan No. 88 []


contact phone: [phone] Fax: 86 () () 86 027-86565322

[after sale service units]
Wuhan De Sheng Chemical Technology Co., Ltd
service hotline: [phone]) (8613397176588)

結果 (英文) 3:

Lithium heparin product manual
"product name" Lithium heparin

"packing" plastic bottle 10G/bottle, 20G/bottle 50g/bottle

"standard" Q/WHDS 0202-2012

"product performance" white amorphous powder, no smell, soluble in water, easy to absorb moisture, average molecular weight is 15,000, stable at room temperature. Our Lithium heparin titer ≥ 150IU/mg, anhydrous titer ≥ 160IU/mg. Other indicators in the light of heparin API standards to control it.
"Main use" this product is intended for in vitro in blood and anticoagulant for clinical blood test additives,In clinical blood collection and testing of blood samples during pretreatment (anticoagulant).

"Scope" this product applies Clinical Biochemistry examination and biochemical test blood specimen collection anticoagulant in emergency, also applies to the part of the blood rheology of blood specimen collection anticoagulant project.
When content in a clinical laboratory blood, Lithium heparin as anticoagulants is recommended, because it is least likely to cause interference on determination of other ions.
This product is not a medicine, not to be used as an injection, prohibits the direct injection of human and animal.
"How" this product with deionized water (distilled water) dissolve,Formulated in certain concentrations and contents of solution, then join in the blood.
A. Solution preparation methods and procedures:
1. go live: electronic scales (one out of 10,000), volumetric flask (100ml, 200ml,500ml,1000ml, based on the need to use), beaker, deionized water (distilled water), glass rods, bottle washing and so on.
2. weighing: weighing electronic balance accurately predetermined number heparin lithium powder, accurate to one out of 10,000, then carefully pour the heparin lithium powder in a volumetric flask or beaker, washing carefully attached to the volumetric flask or beaker and wall of heparin lithium powder into the bottle or Cup;Special attention to the volumetric flasks and beakers are clean, no impurities, grease, bacteria and oxides. Cleaning please refer to the analysts of the volumetric flask and the beaker in the operating manual.
3. dissolution: this product can be used with distilled water, deionized water to dissolve. Can be added with a certain volume of deionized water in a beaker dissolve and mix with water with a glass rod slowly until all dissolved into the volumetric flask. Can also use volumetric flask dissolve. Dissolution to pay particular attention to the dissolution of the beaker of water used in the process control and wanted to give back to clean on a glass rod, beakers and other utensils stained with heparin have adequate storage capacity,This will cause hyper-volume resulting in lower heparin concentration.
4. closed: all Lithium heparin after dissolving, eyes with volumetric flask tick remains horizontal, bottle washing capacity bottle slowly along the wall add deionized water, concave surface with tick marks in a line at the end of May.
5. blending: used corks sealing capacity bottle, hold the volumetric flask in one hand and finger pressure on the plug at the top in one hand and hold the bottom of the volumetric flask, mix carefully in reverse 5-8.
6. checking and validation: carefully check the heparin lithium weights weighing and volume of volumetric flask, and other critical factors, Lithium heparin is recalculated effective content, by the appropriate confirmation.
B. Added to:
1. join the machine: the
(1) cleaning: machine carefully before using the machine, clean the containers and pipes, can be rinsed with deionized water, then 80% concentrations of alcohol containers and pipes 3 times, then use 99% more than ethanol to clean containers and pipelines, with hot-air drying alcohol. To ensure that no impurities, bacteria and water attached to the container and the wall to avoid pollution from Lithium heparin.
(2) adjustment of the machine: the basis of blood volume to be produced determines the number needs to be added to the heparin lithium amount or volume, adjust the flow regulator to the proper value of the machine, add 10-100 test tube,And sampling to join doses to meet the requirements. Solution by adding the allowable error of ± 10%.

2. manual join:
(1) clean containers and tools. Cleaning preparations for loading Lithium heparin solution by container and pipette method with the cleaning of the machine.
(2) distribution of heparin solution and tools: Lithium heparin solution into clean good container, and after cleaning, adjustment to operating personnel in the pipette. Note liquid scale to adjust well to ensure solution volume reach the requirements for joining.
(3) Add liquid: the pipette will join the blood Lithium heparin solution.Confirm the amounts for each operator. Join at any time during random checks. Join the range of allowable error of ± 10%.
C. Baking: you can take hot air blower drying, drying oven, oven drying can also be used. Hot-air drying temperature 50-80 degrees, drying time 10-300 seconds. Fan oven, oven drying at 40-60, 2-6 hours drying time. Drying time drying temperature Lithium heparin solution volume, as well as the fan speed (oven air exhaust capability) and other factors. Special attention to the fan speed should not be too large, so as to avoid Lithium heparin solution were blown out of the blood.
Heparin is usually the best in solution-State, but some users need a dry Lithium heparin tube.

"Additives" in vacuum blood collection tube industry standard recommended dose range for each ml of blood (10USP) -30USP (28IU) units of heparin lithium. Trace blood (peripheral blood) anticoagulant can be less than (15USP) per millilitre of blood. The company recommended a one-time vacuum venous blood collection tube with 15-20IU Lithium heparin anticoagulant ml of blood, peripheral blood anticoagulant 8-10IU ml of anticoagulant.
Heparin potency unit: IU/mg. The effective number of heparin per mg.
Sample tables:Common liver pigment lithium solution preparation and the additives volume table
blood collection tube specifications (ML) 2 3 4 5 6 7 8
liver pigment additives volume (IU) 30 45 60 75 90 105 120
corresponds to of liver pigment lithium weight (mg) 0.2 0.3 0.4 0.5 0.6 0.7 0.8
additives volume (UL) 20 30 40 50 60 70 80
liver pigment solution concentration (g/l) 10 10 10 10 10 10 10
Instructions: 1. amount of additive Lithium heparin add 15IU heparin calculations as per milliliter of blood;
2. Lithium heparin potency in terms 150IU/mg;
3. heparin additive of 15-25IU in the actual production process flexibilityHeparin potency is subject to actual data on the inspection report. Calculated the same way.
4. calculation method: the
(1) corresponds to the weight of Lithium heparin (mg) = amount of additive Lithium heparin (IU)/Lithium heparin real potency (IU/mg).
(2) concentrations (g/l) = weight of Lithium heparin (mg)/additive volume (UL). Special attention to transform units, mg and UL but with g and ml, the percentage of 100ml grams of Lithium heparin into water, final ml into l, you need to multiply the numerator and denominator both 10, draw one per liter needs to join the weight of Lithium heparin.

"Considerations"
 To ensure blood anticoagulant full, heparin tube blood collection must be reversed as soon as possible after the completion of salt mix 5-8, particularly in blood temperature above 25 ° c, mix blood with heparin lithium must be timely and adequate, otherwise easily lead to blood clotting or local blood coagulation.
Non-irreversible anticoagulant heparin and Lithium heparin anticoagulant test tube blood collection should be completed in the 6h test, as it may cause errors with the test results.
Ion and heparin enzyme may be involved in cell metabolism, thus the amount of heparin used may affect the test results. Heparin in the amount of additive should ensure adequate anticoagulation stuffed full and partial,Thus ensuring repeatable in the plasma 6h most of the indicators, in particular, AST, ALT, TBIL, DBIL, GGT and other sensitive targets.
Lithium heparin can be used in conjunction with separation gel, anticoagulant effects, Silicon wall of separation, centrifugation conditions, adhesive quality and can affect a blood separation effect. Proposals are compatible with the company's production of blood separating gel, you can get high quality plasma specimens.
Joined after blood irradiation sterilization recommendations: use of γ-ray irradiation, dose 8-25kGy. Radiation dose can be determined initial number of colonies.

"Warning and prevention"
when Lithium heparin impurity foreign body occurs, a strange odor, color, and when the expiration date is exceeded,The prohibition of the use.
When Lithium heparin solution when there is a haze, Lithium heparin solution may be infection or deterioration, do not continue to use it.
This product as a biological agent, safe, non-toxic, harmless.

"Heparin principle" of heparin from animal extracts in intestinal mucosa contains a Mucopolysaccharide sulfuric acid groups, is a bio-active substances with strong negative charge, heparin activation of Antithrombin ⅲ in the blood serine protease inactivation, thus preventing the formation of Thrombin and prevent platelet aggregation, and other anticoagulant effect. It have a bearing on three stages of the process of blood coagulation, anticoagulant effects in both in vitro and in vivo, can extend the clotting time,Prothrombin and Thrombin time.

"Instructions or diagram" Please read this manual carefully before use. Is not clear please contact with our company.

"Maintenance product" does not apply.

"Storage conditions" this product can keep tightly in cool and dry place, protected from light, no moisture. Aqueous solution of the product can be 0-4 ℃ sealed in a sterile State, the longest retention period is not more than 7 days. Recommends this product of the blending, with aqueous, solution State not to long time storage.

"Validity period" three years

"other matters", common units conversion
1. weight units: 1g=1000 mg;1kg=1000 g;
2.Unit size: 1ml=1000 UL,1l=1000 ml;
3. efficiency unit: 1IU=1.064 USP.

"Production Enterprise name" Wuhan de Sheng chemical technology limited

"registered address" Wuhan Lake sights Gong home Ridge abalone home Bay 88th,

"production address" Wuhan Lake sights Gong home Ridge abalone home Bay 88th,

"contact way"
phone: () 86 027-86576982 fax: () 86 027-86565322

"after-sales service units"
Wuhan de Sheng chemical technology limited
service line: () 86 02786565322 ( ) 86