通过对滞绿基因研究发现，番茄gf 突变体有滞绿的表现，并且已有人对番茄

通过对滞绿基因研究发现，番茄gf 突变体有滞绿的表现，并且已有人对番茄滞绿基因LeSGR1 进行了克隆，并研究了其功能[36]，也有研究豌豆中的滞绿基因，并对其克隆和分析其功能[37]，但是SGR基因是如何产生滞绿这一表现的，目前并不清楚。研究表明，植物激素中ABA这种激素对植物的衰老有明显的作用，而滞绿基因SGR 的超量表达也会引起植物叶片的衰老，其中SGR 基因的滞绿原因是否通过控制ABA 合成过程中重要酶的合成来间接影响植物的衰老，这对探索SGR基因的滞绿机理有重要意义。本文以番茄为实验材料，探寻SGR 基因在衰老调控与ABA 的代谢控制衰老之间的关系。通过对SGR 转基因植株、RanBP3 转基因植株以及对照A57 分别进行外源ABA、ABA 抑制剂、水喷施处理，然后对不同处理的植株叶片进行ABA含量、ABA 相关基因表达量、生理生化指标的检测。

結果 (英文) 1:

Through on delay green gene research found, tomato GF mutation body has delay green of performance, and has was on tomato delay green gene LeSGR1 for has clone, and research has its function [36], also has research pea in the of delay green gene, and on its clone and analysis its function [37], but SGR gene is how produced delay green this a performance of, currently does not clear. Research has shown that plant hormones ABA which had obvious effects on plant senescence, but excess of SGR-green gene expression can also cause leaf senescence in plants, the SGR gene-green cause is through the control of ABA biosynthesis to indirectly influence the synthesis of important enzymes in plant senescence, which is important for exploring the SGR mechanism of gene-green. Based on tomato experiment, explore the SGR gene in aging control and control the relationship between aging of ABA metabolism. By SGR, RanBP3 transgenic plants transgenic plants as well as exogenous control A57 ABA, ABA inhibitors, water spraying treatment and ABA of leaves of different plants content, expression of genes related to ABA, physiological and biochemical indexes detection.

結果 (英文) 2:

By Lag green gene study found that tomato gf mutant has a stay-green performance, and have someone tomato stagnant green gene LeSGR1 cloned and studied its function [36], there are also studies peas stagnation green gene, and cloning and functional analysis of its [37], but the SGR gene is how to produce the green stagnant performance, is not clear. Studies have shown that the plant hormone ABA hormone of aging plants have significant effects, whereas overexpression of stagnant green SGR gene can also cause plant leaf senescence, in which stagnant green whether the reasons SGR gene synthesis process by controlling the ABA synthesizing enzyme important to the indirect effects of aging plants, which explore the SGR gene stagnant green mechanism is important. In this paper, Tomato as experimental material to explore SGR gene in the metabolic regulation of aging and ABA control relationship between senescence. By SGR transgenic plants, RanBP3 transgenic plants and control A57 were exogenous ABA, ABA inhibitors, water spraying process, and then the plants leaves of different processing will be ABA content detected related gene expression of ABA, physiological and biochemical indexes.

結果 (英文) 3:

It was discovered through research on green gene GF in tomato mutant with lag lag, green performance, and the people were cloned in tomato stay green gene LeSGR1, and to study the function of [36], there are studies in peas stay green gene, and the cloning and analysis of the function of [37], but the SGR gene is how to produce stay green this performance, it is not clear. The research shows that there is obvious effect of this hormone in aging ABA plant hormones on plants, excessive stay green gene SGR expression can cause leaf senescence, whether the SGR gene causes by enzyme control lag green synthesis process of ABA synthesis to influence plant senescence indirectly, which is of great significance to explore the mechanism of SGR gene in stay green. In this paper, we explore the relationship between the senescence of SGR gene and the senescence of ABA by using tomato as the experimental material. Based on the SGR transgenic plants and RanBP3 transgenic plants and control A57 were exogenous ABA, ABA inhibitor, water spraying, and then leaves of different treatments of ABA content and ABA gene expression detection, physiological and biochemical indexes.